The automation friendly protocol minimizes hands on time to generate sequencer ready stranded indexed libraries depleted of rrna and globin in as little as 3 5 hours.
Rna seq library prep kit.
Please note that adaptors primers rrna depletion reagents and poly a mrna isolation reagents are not included in the kit and are available separately.
Zymo seq ribofree total rna seq library kit is the fastest easiest rna seq library prep kit available to make stranded total rna libraries.
095 117 119 132 134 except bundles with ribocop rrna depletion kits cat.
Truseq rna sample preparation v2 high sample experienced user card and lab tracking form documentation.
Sequencing library qpcr quantification guide documentation.
Truseq rna library prep kit v2 documentation.
The nebnext ultra rna library prep kit for illumina contains reagents for preparation of non directional rna libraries for illumina sequencing.
Truseq sample prep best practices guide documentation.
146 147 and applies for direct customers only.
This offer is valid until the end of 2020 or while stock lasts.
The invitrogen collibri 3 mrna library prep kits for illumina systems capture the 3 end of the transcript enabling gene expression studies from as little as 2 million reads per sample compared to 60 million reads for whole transcriptome sequencing or 30 million reads for mrna sequencing.
Custom protocol selector generates customized end to end instructions.
Poly a containing mrna was isolated from 10 ng 100 ng and 1 µg of universal human reference rna agilent 740000 and libraries were made using the nebnext ultra ii directional rna kit kapa stranded mrna seq kit kapa mrna hyperprep kit and illumina truseq stranded mrna kit.